IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.

IL-10 reduces levels of apoptosis in Toxoplasma gondii-infected trophoblasts.

Blog Article

BACKGROUND: To analyze the effects of IL-10 on the HLA-G expression and the apoptosis of trophoblasts infected with Toxoplasma gondii.METHODS: T.gondii-infected or uninfected human trophoblasts and immortalized human placental BeWo cells were cultured with or without human IL-10.Uninfected and infected cells without IL-10 cells served as controls.

HLA-G expression was measured by real-time PCR and flow cytometry, respectively.Cells apoptosis were analyzed by flow cytometry.Apoptosis associated moleculars were measured by real-time PCR and Western bolt.RESULTS: HLA-G expression was increased Packaging in the infected trophoblasts and BeWo cells compared to uninfected cells.

Treatment of infected cells with IL-10 decreased HLA-G expression compared to Tinned Soups infected cells while no change in treatment of uninfected cells compared with uninfected cells.Levels of apoptosis and apoptosis associated caspase-3 and caspase-8 decreased and c-FLIP levels increased in treated infected cells with IL-10 compared to infected cells and no difference in IL-10 treated uninfected cells compared to uninfected cells.CONCLUSIONS: IL-10 regulates HLA-G expression in T.gondii-infected trophoblasts.

IL-10 treatment of infected trophoblasts reduced levels of apoptosis.This may contribute to the improvement in pregnancy outcomes when women infected with T.gondii treated with IL-10.